Surface Inoculation and Quantification of Pseudomonas syringae Population in the Arabidopsis Leaf Apoplast.
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ABSTRACT:
SUBMITTER: Jacob C
PROVIDER: S-EPMC5448416 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Surface Inoculation and Quantification of <i>Pseudomonas syringae</i> Population in the <i>Arabidopsis</i> Leaf Apoplast.
Jacob Cristián C Panchal Shweta S Melotto Maeli M
Bio-protocol 20170301 5
Bacterial pathogens must enter the plant tissue in order to cause a successful infection. Foliar bacterial pathogens that are not able to directly penetrate the plant epidermis rely on wounds or natural openings to internalize leaves. This protocol describes a procedure to estimate the population size of <i>Pseudomonas syringae</i> in the leaf apoplast after surface inoculation of <i>Arabidopsis</i> rosettes. ...[more]
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