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Production of biodiesel from microalgae through biological carbon capture: a review.

ABSTRACT: Gradual increase in concentration of carbon dioxide (CO₂) in the atmosphere due to the various anthropogenic interventions leading to significant alteration in the global carbon cycle has been a subject of worldwide attention and matter of potential research over the last few decades. In these alarming scenario microalgae seems to be an attractive medium for capturing the excess CO₂ present in the atmosphere generated from different sources such as power plants, automobiles, volcanic eruption, decomposition of organic matters and forest fires. This captured CO₂ through microalgae could be used as potential carbon source to produce lipids for the generation of biofuel for replacing petroleum-derived transport fuel without affecting the supply of food and crops. This comprehensive review strives to provide a systematic account of recent developments in the field of biological carbon capture through microalgae for its utilization towards the generation of biodiesel highlighting the significance of certain key parameters such as selection of efficient strain, microalgal metabolism, cultivation systems (open and closed) and biomass production along with the national and international biodiesel specifications and properties. The potential use of photobioreactors for biodiesel production under the influence of various factors viz., light intensity, pH, time, temperature, CO₂ concentration and flow rate has been discussed. The review also provides an economic overview and future outlook on biodiesel production from microalgae.

SUBMITTER: Mondal M

PROVIDER: S-EPMC5449280 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Production of biodiesel from microalgae through biological carbon capture: a review.

Mondal Madhumanti M Goswami Shrayanti S Ghosh Ashmita A Oinam Gunapati G Tiwari O N ON Das Papita P Gayen K K Mandal M K MK Halder G N GN

3 Biotech 20170530 2

Gradual increase in concentration of carbon dioxide (CO<sub>2</sub>) in the atmosphere due to the various anthropogenic interventions leading to significant alteration in the global carbon cycle has been a subject of worldwide attention and matter of potential research over the last few decades. In these alarming scenario microalgae seems to be an attractive medium for capturing the excess CO<sub>2</sub> present in the atmosphere generated from different sources such as power plants, automobiles ...[more]

PMID: 28560639

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Project description:BACKGROUND:We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the enzyme activity and amount of enzyme produced were not adequate for industrial application. Our goal in the present study was to improve the enzyme properties of RML in order to apply it for the conversion of microalgae oil to biofuel. RESULTS:Several new expression plasmids were constructed by adding the propeptide of the target gene, optimizing the signal peptide, and varying the number of target gene copies. Each plasmid was transformed separately into P. pastoris strain X-33. Screening by flask culture showed maximal (21.4-fold increased) enzyme activity for the recombinant strain with two copies of the target gene; the enzyme was termed Lipase GH2. The expressed protein with the propeptide (pRML) was a stable glycosylated protein, because of glycosylation sites in the propeptide. Quantitative real-time RT-PCR analysis revealed two major reasons for the increase in enzyme activity: (1) the modified recombinant expression system gave an increased transcription level of the target gene (rml), and (2) the enzyme was suitable for expression in host cells without causing endoplasmic reticulum (ER) stress. The modified enzyme had improved thermostability and methanol or ethanol tolerance, and was applicable directly as free lipase (fermentation supernatant) in the catalytic esterification and transesterification reaction. After reaction for 24 hours at 30°C, the conversion rate of microalgae oil to biofuel was above 90%. CONCLUSIONS:Our experimental results show that signal peptide optimization in the expression plasmid, addition of the gene propeptide, and proper gene dosage significantly increased RML expression level and enhanced the enzymatic properties. The target enzyme was the major component of fermentation supernatant and was stable for over six months at 4°C. The modified free lipase is potentially applicable for industrial-scale conversion of microalgae oil to biodiesel.

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Production of biodiesel from microalgae through biological carbon capture: a review.

Publications

Production of biodiesel from microalgae through biological carbon capture: a review.

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