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Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro.

ABSTRACT: Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In this study, we developed a new class of folate-functionalized, superparamagnetic iron oxide (SPIO)-loaded cationic amylose nanoparticles to deliver survivin-siRNA to HCC cells. The cellular uptake of nanocomplexes, cytotoxicity, cell apoptosis, and gene suppression mediated by siRNA-complexed nanoparticles were tested. The results demonstrated that folate-functionalized, SPIO-loaded cationic amylose nanoparticles can mediate a specific and safe cellular uptake of survivin-siRNA with high transfection efficiency, resulting in a robust survivin gene downregulation in HCC cells. The biocompatible complex of cationic amylose could be used as an efficient, rapid, and safe gene delivery vector. Upon SPIO loading, it holds a great promise as a theranostic carrier for gene therapy of HCC.

SUBMITTER: Wu Z

PROVIDER: S-EPMC5449991 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Phytogenic magnetic nanoparticles (PMNPs) were fabricated using plant leaves' extract of Fraxinus chinensis Roxb. and then, the surfaces of the PMNPs were functionalized by 3-mercaptopropionic acid (3-MPA) to investigate the adsorptive removal of the toxic dye malachite green (MG) from aqueous solutions. The preparation and coating of 3-MPA on the surface of the PMNPs was confirmed and characterized using different techniques, which are UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), scanning electron microscopy with integrated energy dispersive X-ray analysis (SEM-EDX), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM), Brunauer-Emmett-Teller (BET) analysis and thermogravimetric analysis (TGA). The hysteresis loops of 3-MPA@PMNPs depicted an excellent superparamagnetic nature with saturation magnetization values of 50.95 emu g-1. The prepared material showed the highest adsorptive rate (98.57% MG removal within 120 min) and an estimated comparable adsorptive capacity of 81.2 mg g-1 at 25 °C. The experimental data were well fitted to the Langmuir isotherm, indicating the monolayer adsorption of MG onto 3-MPA@PMNPs. Furthermore, the kinetic data agreed well with the pseudo-second-order model, indicating the removal of MG by chemisorption and/or ion-exchange mechanism. Thermodynamic study confirmed that the adsorption of MG was exothermic and spontaneous. The high adsorptive removal of the dye not only persisted over a wide pH range (6-12), but the material also demonstrated high selectivity in the presence of co-existing ions (i.e. Pd2+ and Cd2+) along with the fastest separation times (35 s) from aqueous solutions. The recovered adsorbent (3-MPA@PMNPs) was reused five times and maintained a removal efficiency of more than 85%. Therefore, the prepared novel 3-MPA@PMNPs can be employed as an alternative low-cost sorbent material for the removal cationic dyes from textile wastewater. In addition, this green nanotechnology/strategy can easily be implemented in low-economy countries for wastewater treatment.

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Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro.

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