High-quality genetic mapping with ddRADseq in the non-model tree Quercus rubra.

ABSTRACT:

Background

Restriction site associated DNA sequencing (RADseq) has the potential to be a broadly applicable, low-cost approach for high-quality genetic linkage mapping in forest trees lacking a reference genome. The statistical inference of linear order must be as accurate as possible for the correct ordering of sequence scaffolds and contigs to chromosomal locations. Accurate maps also facilitate the discovery of chromosome segments containing allelic variants conferring resistance to the biotic and abiotic stresses that threaten forest trees worldwide. We used ddRADseq for genetic mapping in the tree Quercus rubra, with an approach optimized to produce a high-quality map. Our study design also enabled us to model the results we would have obtained with less depth of coverage.

SUBMITTER: Konar A 

PROVIDER: S-EPMC5450186 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature