Ontology highlight
ABSTRACT:
SUBMITTER: Chen JWC
PROVIDER: S-EPMC5450317 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Chen Jack W C JWC Chen Zhuo A ZA Rogala Kacper B KB Metz Jeremy J Deane Charlotte M CM Rappsilber Juri J Wakefield James G JG
Biology open 20170515 5
The hetero-octameric protein complex, Augmin, recruits γ-Tubulin ring complex (γ-TuRC) to pre-existing microtubules (MTs) to generate branched MTs during mitosis, facilitating robust spindle assembly. However, despite a recent partial reconstitution of the human Augmin complex <i>in vitro</i>, the molecular basis of this recruitment remains unclear. Here, we used immuno-affinity purification of <i>in vivo</i> Augmin from <i>Drosophila</i> and cross-linking/mass spectrometry to identify distance ...[more]