Unknown

Dataset Information

0

DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation.


ABSTRACT: Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.

SUBMITTER: Moon EK 

PROVIDER: S-EPMC5450953 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

DNA Methylation of Gene Expression in <i>Acanthamoeba castellanii</i> Encystation.

Moon Eun-Kyung EK   Hong Yeonchul Y   Lee Hae-Ahm HA   Quan Fu-Shi FS   Kong Hyun-Hee HH  

The Korean journal of parasitology 20170430 2


Encystation mediating cyst specific cysteine proteinase (CSCP) of <i>Acanthamoeba castellanii</i> is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of <i>Acanthamoeba</i>. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between pr  ...[more]

Similar Datasets

| S-EPMC4468156 | biostudies-literature
| S-EPMC7376869 | biostudies-literature
| S-EPMC7835678 | biostudies-literature
| S-EPMC4206453 | biostudies-literature
| PRJNA286088 | ENA
| PRJNA12731 | ENA
| PRJNA422304 | ENA
| PRJNA772372 | ENA
| PRJNA12783 | ENA
| PRJNA1195399 | ENA