Dataset Information

Methylmercury uptake and degradation by methanotrophs.

ABSTRACT: Methylmercury (CH₃Hg⁺) is a potent neurotoxin produced by certain anaerobic microorganisms in natural environments. Although numerous studies have characterized the basis of mercury (Hg) methylation, no studies have examined CH₃Hg⁺ degradation by methanotrophs, despite their ubiquitous presence in the environment. We report that some methanotrophs, such as Methylosinus trichosporium OB3b, can take up and degrade CH₃Hg⁺ rapidly, whereas others, such as Methylococcus capsulatus Bath, can take up but not degrade CH₃Hg⁺. Demethylation by M. trichosporium OB3b increases with increasing CH₃Hg⁺ concentrations but was abolished in mutants deficient in the synthesis of methanobactin, a metal-binding compound used by some methanotrophs, such as M. trichosporium OB3b. Furthermore, addition of methanol (>5 mM) as a competing one-carbon (C1) substrate inhibits demethylation, suggesting that CH₃Hg⁺ degradation by methanotrophs may involve an initial bonding of CH₃Hg⁺ by methanobactin followed by cleavage of the C-Hg bond in CH₃Hg⁺ by the methanol dehydrogenase. This new demethylation pathway by methanotrophs indicates possible broader involvement of C1-metabolizing aerobes in the degradation and cycling of toxic CH₃Hg⁺ in the environment.

SUBMITTER: Lu X

PROVIDER: S-EPMC5451197 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Methylmercury uptake and degradation by methanotrophs.

Lu Xia X Gu Wenyu W Zhao Linduo L Farhan Ul Haque Muhammad M DiSpirito Alan A AA Semrau Jeremy D JD Gu Baohua B

Science advances 20170531 5

Methylmercury (CH3Hg+) is a potent neurotoxin produced by certain anaerobic microorganisms in natural environments. Although numerous studies have characterized the basis of mercury (Hg) methylation, no studies have examined CH3Hg+ degradation by methanotrophs, despite their ubiquitous presence in the environment. We report that some methanotrophs, such as Methylosinus trichosporium OB3b, can take up and degrade CH3Hg+ rapi ...[more]

PMID: 28580426

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Project description:Many studies have recognized abiotic photochemical degradation as an important sink of methylmercury (CH3Hg) in sunlit surface waters, but the rate-controlling factors remain poorly understood. The overall objective of this study was to improve our understanding of the relative importance of photochemical reactions in the degradation of CH3Hg in surface waters across a variety of marine ecosystems by extending the range of water types studied. Experiments were conducted using surface water collected from coastal sites in Delaware, New Jersey, Connecticut, and Maine, as well as offshore sites on the New England continental shelf break, the equatorial Pacific, and the Arctic Ocean. Filtered water amended with additional CH3Hg at environmentally relevant concentrations was allowed to equilibrate with natural ligands before being exposed to natural sunlight. Water quality parameters - salinity, dissolved organic carbon, and nitrate - were measured, and specific UV absorbance was calculated as a proxy for dissolved aromatic carbon content. Degradation rate constants (0.87-1.67 day-1) varied by a factor of two across all water types tested despite varying characteristics, and did not correlate with initial CH3Hg concentrations or other environmental parameters. The rate constants in terms of cumulative photon flux values were comparable to, but at the high end of, the range of values reported in other studies. Further experiments investigating the controlling parameters of the reaction observed little effect of nitrate and chloride, and potential for bromide involvement. The HydroLight radiative transfer model was used to compute solar irradiance with depth in three representative water bodies - coastal wetland, estuary, and open ocean - allowing for the determination of water column integrated rates. Methylmercury loss per year due to photodegradation was also modeled across a range of latitudes from the Arctic to the Equator in the three model water types, resulting in an estimated global demethylation rate of 25.3 Mmol yr-1. The loss of CH3Hg was greatest in the open ocean due to increased penetration of all wavelengths, especially the UV portion of the spectrum which has a greater ability to degrade CH3Hg. Overall, this study provides additional insights and information to better constrain the importance of photochemical degradation in the cycling of CH3Hg in marine surface waters and its transport from coastal waters to the open ocean.

Dataset Information

Methylmercury uptake and degradation by methanotrophs.

Publications

Methylmercury uptake and degradation by methanotrophs.

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