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Cooperation between a hierarchical set of recruitment sites targets the X chromosome for dosage compensation.


ABSTRACT: In many organisms, it remains unclear how X chromosomes are specified for dosage compensation, since DNA sequence motifs shown to be important for dosage compensation complex (DCC) recruitment are themselves not X-specific. Here, we addressed this problem in C. elegans. We found that the DCC recruiter, SDC-2, is required to maintain open chromatin at a small number of primary DCC recruitment sites, whose sequence and genomic context are X-specific. Along the X, primary recruitment sites are interspersed with secondary sites, whose function is X-dependent. A secondary site can ectopically recruit the DCC when additional recruitment sites are inserted either in tandem or at a distance (>30 kb). Deletion of a recruitment site on the X results in reduced DCC binding across several megabases surrounded by topologically associating domain (TAD) boundaries. Our work elucidates that hierarchy and long-distance cooperativity between gene-regulatory elements target a single chromosome for regulation.

SUBMITTER: Albritton SE 

PROVIDER: S-EPMC5451215 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Cooperation between a hierarchical set of recruitment sites targets the X chromosome for dosage compensation.

Albritton Sarah Elizabeth SE   Kranz Anna-Lena AL   Winterkorn Lara Heermans LH   Street Lena Annika LA   Ercan Sevinc S  

eLife 20170530


In many organisms, it remains unclear how X chromosomes are specified for dosage compensation, since DNA sequence motifs shown to be important for dosage compensation complex (DCC) recruitment are themselves not X-specific. Here, we addressed this problem in <i>C. elegans</i>. We found that the DCC recruiter, SDC-2, is required to maintain open chromatin at a small number of primary DCC recruitment sites, whose sequence and genomic context are X-specific. Along the X, primary recruitment sites a  ...[more]

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