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CCAAT/enhancer-binding protein ? decreases the viability of gastric cancer cells.

ABSTRACT: CCAAT/enhancer-binding protein (C/EBP) ?, C/EBP? and C/EBP? are involved in inflammation and cell differentiation. In the present study, their roles in human gastric cancer cells were investigated. The human gastric cancer cell lines MKN45 and MKN74 were subjected to the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the expression levels of C/EBP?, C/EBP? and C/EBP?. The cells were transfected with expression plasmids for either C/EBP? or C/EBP?, and subjected to a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay and RT-qPCR for analysis of cyclin D1 expression. Expression levels of C/EBP? and C/EBP? were decreased in MKN45 and MKN74 cells compared with in normal gastric tissue. Expression levels of C/EBP? were decreased in MKN45 cells and increased in MKN74 cells. Viability of MKN45 cells was decreased by C/EBP? and C/EBP?. Viability of MKN74 cells was decreased by C/EBP?, but increased by C/EBP?. Expression levels of cyclin D1 were decreased in association with C/EBP? and C/EBP? overexpression in MKN45 cells. Expression levels of cyclin D1 were decreased in association with C/EBP? overexpression, but increased in association with C/EBP? overexpression, in MKN74 cells. The results of the present study indicate that C/EBP? is potentially useful for the treatment of gastric cancer.

SUBMITTER: Tomizawa M 

PROVIDER: S-EPMC5453173 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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CCAAT/enhancer-binding protein α decreases the viability of gastric cancer cells.

Tomizawa Minoru M   Shinozaki Fuminobu F   Motoyoshi Yasufumi Y   Sugiyama Takao T   Yamamoto Shigenori S   Ishige Naoki N  

Oncology letters 20170405 6

CCAAT/enhancer-binding protein (C/EBP) α, C/EBPβ and C/EBPδ are involved in inflammation and cell differentiation. In the present study, their roles in human gastric cancer cells were investigated. The human gastric cancer cell lines MKN45 and MKN74 were subjected to the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the expression levels of C/EBPα, C/EBPβ and C/EBPδ. The cells were transfected with expression plasmids for either C/EBPα or C/EBPδ, and subjected  ...[more]

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