C-terminal splice variants of P/Q-type Ca²⁺ channel Ca_V2.1 α₁ subunits are differentially regulated by Rab3-interacting molecule proteins.

ABSTRACT: Voltage-dependent Ca²⁺ channels (VDCCs) mediate neurotransmitter release controlled by presynaptic proteins such as the scaffolding proteins Rab3-interacting molecules (RIMs). RIMs confer sustained activity and anchoring of synaptic vesicles to the VDCCs. Multiple sites on the VDCC α₁ and β subunits have been reported to mediate the RIMs-VDCC interaction, but their significance is unclear. Because alternative splicing of exons 44 and 47 in the P/Q-type VDCC α₁ subunit Ca_V2.1 gene generates major variants of the Ca_V2.1 C-terminal region, known for associating with presynaptic proteins, we focused here on the protein regions encoded by these two exons. Co-immunoprecipitation experiments indicated that the C-terminal domain (CTD) encoded

SUBMITTER: Hirano M 

PROVIDER: S-EPMC5454116 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature