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Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida.

ABSTRACT: Gerbera hybrida is a cut-flower crop of global importance, and an understanding of the mechanisms underlying petal development is vital for the continued commercial development of this plant species. Brassinosteroids (BRs), a class of phytohormones, are known to play a major role in cell expansion, but their effect on petal growth in G. hybrida is largely unexplored. In this study, we found that the brassinolide (BL), the most active BR, promotes petal growth by lengthening cells in the middle and basal regions of petals, and that this effect on petal growth was greater than that of gibberellin (GA). The RNA-seq (high-throughput cDNA sequencing) technique was employed to investigate the regulatory mechanisms by which BRs control petal growth. A global transcriptome analysis of the response to BRs in petals was conducted and target genes regulated by BR were identified. These differentially expressed genes (DEGs) include various transcription factors (TFs) that were activated during the early stage (0.5 h) of BL treatment, as well as cell wall proteins whose expression was regulated at a late stage (10 h). BR-responsive DEGs are involved in multiple plant hormone signal pathways, hormone biosynthesis and biotic and abiotic stress responses, showing that the regulation of petal growth by BRs is a complex network of processes. Thus, our study provides new insights at the transcriptional level into the molecular mechanisms of BR regulation of petal growth in G. hybrida.

SUBMITTER: Huang G

PROVIDER: S-EPMC5455292 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida.

Huang Gan G Han Meixiang M Yao Wei W Wang Yaqin Y

PeerJ 20170531

Gerbera hybrida is a cut-flower crop of global importance, and an understanding of the mechanisms underlying petal development is vital for the continued commercial development of this plant species. Brassinosteroids (BRs), a class of phytohormones, are known to play a major role in cell expansion, but their effect on petal growth in G. hybrida is largely unexplored. In this study, we found that the brassinolide (BL), the most active BR, promotes petal growth by lengthening cells i ...[more]

PMID: 28584713

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Project description:BackgroundGerbera hybrida is one of the most popular cut flowers in the world; however, stem bending, which always happens when gerbera flower harvested from the field, greatly limits its vase life. To date the molecular mechanisms underlying stem bending remain poorly understood.ResultsIn this study, we performed high-throughput transcriptome sequencing of gerbera during stem bending using the Illumina sequencing technology. Three cDNA libraries constructed from mRNAs of gerbera stem at stem bending stage 0, 2 and 4 were sequenced. More than 300 million high-quality reads were generated and assembled into 96,492 unigenes. Among them, 34,166 unigenes were functionally annotated based on similarity search with known protein. Sequences derived from plants at different stem bending stages were mapped to the assembled transcriptome, and 9,406 differentially expressed genes (DEGs) were identified. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, specific pathways were identified during the stem bending process, such as phenylpropanoid biosynthesis pathway, phenylalanine metabolism pathway, starch and sucrose metabolism pathway, and plant hormone signal transduction pathway. A total of 211 transcription factors (TFs), including TF families involved in plant senescence, such as NAC, MYB, WRKY, and AP2/ERF members, as well as TFs related to water stress tolerance, were shown to be regulated during stem bending. Gene Onotology (GO) functional enrichment analysis indicated that key genes involved in responses to osmotic and oxidative stresses were also varied in expression during this process. Furthermore, analysis of DEGs involved in the hormone signaling pathways and determination of endogenous abscisic acid (ABA) content showed that stem bending may be an ethylene-independent process, but regulated by ABA. In short, our findings suggested that the stem bending of cut gerbera may be caused by the involvement of water stress and regulation of ABA during the postharvest life.ConclusionsThe transcriptome sequences provide a valuable resource in revealing the molecular mechanism underlying stem bending of cut flower and offer novel genes that can be used to guide future studies for ornamental plant breeding.

Dataset Information

Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in Gerbera hybrida.

Publications

Transcriptome analysis reveals the regulation of brassinosteroids on petal growth in <i>Gerbera hybrida</i>.

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