Genome-wide mutagenesis of Zea mays L. using RescueMu transposons.
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ABSTRACT:
SUBMITTER: Fernandes J
PROVIDER: S-EPMC545602 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Publications
Genome-wide mutagenesis of Zea mays L. using RescueMu transposons.
Fernandes John J Dong Qunfeng Q Schneider Bret B Morrow Darren J DJ Nan Guo-Ling GL Brendel Volker V Walbot Virginia V
Genome biology 20040923 10
Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots re ...[more]
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