Hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal proteins.

ABSTRACT:

Background

Can sequence segments coding for subcellular targeting or for posttranslational modifications occur in proteins that are not substrates in either of these processes? Although considerable effort has been invested in achieving low false-positive prediction rates, even accurate sequence-analysis tools for the recognition of these motifs generate a small but noticeable number of protein hits that lack the appropriate biological context but cannot be rationalized as false positives.

Results

We show that the carboxyl termini of a set of definitely non-peroxisomal proteins with predicted peroxisomal targeting signals interact with the peroxisomal matrix protein receptor peroxin 5 (PEX5) in a yeast two-hybrid test. Moreover, we show that examples of these proteins - chic

SUBMITTER: Neuberger G 

PROVIDER: S-EPMC545800 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature