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Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells.


ABSTRACT: Adipose stromal cells (ASCs) have been identified as a mesenchymal cell population recruited from white adipose tissue (WAT) by tumors and supporting cancer progression. We have previously reported the existence of a non-glycanated decorin isoform (ngDCN) marking mouse ASCs. We identified a peptide CSWKYWFGEC that binds to ngDCN and hence can serve as a vehicle for ASC-directed therapy delivery. We used hunter-killer peptides composed of CSWKYWFGEC and a pro-apoptotic moiety to deplete ASCs and suppress growth of mouse tumors. Here, we report the discovery of the human non-glycanated decorin isoform. We show that CSWKYWFGEC can be used as a probe to identify ASCs in human WAT and tumors. We demonstrate that human ngDCN is expressed on ASC surface. Finally, we validate ngDCN as a molecular target for pharmacological depletion of human ASCs with hunter-killer peptides. We propose that ngDCN-targeting agents could be developed for obesity and cancer treatment.

SUBMITTER: Daquinag AC 

PROVIDER: S-EPMC5458115 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells.

Daquinag Alexes C AC   Dadbin Ali A   Snyder Brad B   Wang Xiaoping X   Sahin Aysegul A AA   Ueno Naoto T NT   Kolonin Mikhail G MG  

Molecular therapy oncolytics 20170517


Adipose stromal cells (ASCs) have been identified as a mesenchymal cell population recruited from white adipose tissue (WAT) by tumors and supporting cancer progression. We have previously reported the existence of a non-glycanated decorin isoform (ngDCN) marking mouse ASCs. We identified a peptide CSWKYWFGEC that binds to ngDCN and hence can serve as a vehicle for ASC-directed therapy delivery. We used hunter-killer peptides composed of CSWKYWFGEC and a pro-apoptotic moiety to deplete ASCs and  ...[more]

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