Targeted recombination between homologous chromosomes for precise breeding in tomato.
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ABSTRACT:
SUBMITTER: Filler Hayut S
PROVIDER: S-EPMC5458649 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Targeted recombination between homologous chromosomes for precise breeding in tomato.
Filler Hayut Shdema S Melamed Bessudo Cathy C Levy Avraham A AA
Nature communications 20170526
Homologous recombination (HR) between parental chromosomes occurs stochastically. Here, we report on targeted recombination between homologous chromosomes upon somatic induction of DNA double-strand breaks (DSBs) via CRISPR-Cas9. We demonstrate this via a visual and molecular assay whereby DSB induction between two alleles carrying different mutations in the PHYTOENE SYNTHASE (PSY1) gene results in yellow fruits with wild type red sectors forming via HR-mediated DSB repair. We also show that in ...[more]
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