Ontology highlight
ABSTRACT:
SUBMITTER: Blondal T
PROVIDER: S-EPMC546137 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Blondal Thorarinn T Thorisdottir Audur A Unnsteinsdottir Unnur U Hjorleifsdottir Sigridur S Aevarsson Arnthor A Ernstsson Sveinn S Fridjonsson Olafur H OH Skirnisdottir Sigurlaug S Wheat Jon Oskar JO Hermannsdottir Anna Gudny AG Sigurdsson Snorri Th ST Hreggvidsson Gudmundur O GO Smith Albert Vernon AV Kristjansson Jakob K JK
Nucleic acids research 20050107 1
We have recently sequenced the genome of a novel thermophilic bacteriophage designated as TS2126 that infects the thermophilic eubacterium Thermus scotoductus. One of the annotated open reading frames (ORFs) shows homology to T4 RNA ligase 1, an enzyme of great importance in molecular biology, owing to its ability to ligate single-stranded nucleic acids. The ORF was cloned, and recombinant protein was expressed, purified and characterized. The recombinant enzyme ligates single-stranded nucleic a ...[more]