Ontology highlight
ABSTRACT:
SUBMITTER: Sjoholm J
PROVIDER: S-EPMC5462737 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Sjöholm Johannes J Bergstrand Jan J Nilsson Tobias T Šachl Radek R Ballmoos Christoph von CV Widengren Jerker J Brzezinski Peter P
Scientific reports 20170607 1
We have investigated the effect of lipid composition on interactions between cytochrome bo <sub>3</sub> and ATP-synthase, and the ATP-synthesis activity driven by proton pumping. The two proteins were labeled by fluorescent probes and co-reconstituted in large (d ≅ 100 nm) or giant (d ≅ 10 µm) unilamellar lipid vesicles. Interactions were investigated using fluorescence correlation/cross-correlation spectroscopy and the activity was determined by measuring ATP production, driven by electron-prot ...[more]