Inhibition of glioblastoma tumorspheres by combined treatment with 2-deoxyglucose and metformin.

ABSTRACT:

Background

Deprivation of tumor bioenergetics by inhibition of multiple energy pathways has been suggested as an effective therapeutic approach for various human tumors. However, this idea has not been evaluated in glioblastoma (GBM). We hypothesized that dual inhibition of glycolysis and oxidative phosphorylation could effectively suppress GBM tumorspheres (TS).

Methods

Effects of 2-deoxyglucose (2DG) and metformin, alone and in combination, on GBM-TS were evaluated. Viability, cellular energy metabolism status, stemness, invasive properties, and GBM-TS transcriptomes were examined. In vivo efficacy was tested in a mouse orthotopic xenograft model.

Results

GBM-TS viability was decreased by the combination of 2DG and metformin. ATP assay and PET showed that cellular e

SUBMITTER: Kim EH 

PROVIDER: S-EPMC5464255 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature