A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
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ABSTRACT:
SUBMITTER: MacDonald RB
PROVIDER: S-EPMC5466054 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Publications
A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
MacDonald Ryan B RB Kashikar Nachiket D ND Lagnado Leon L Harris William A WA
Zebrafish 20161227 3
Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated wi ...[more]
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