MiR-34b attenuates trauma-induced anxiety-like behavior by targeting CRHR1.

ABSTRACT: Exposure to trauma is a potential contributor to anxiety; however, the molecular mechanisms responsible for trauma-induced anxiety require further clarification. In this study, in an aim to explore these mechanisms, we observed the changes in the hypothalamic pituitary adrenal (HPA) axis using a radioimmunoassay and the changes in anxiety-like behavior using the open field test and elevated plus maze test in a rat model following intervention with NBI?27914, a specific corticotropin?releasing hormone receptor 1 (CRHR1) antagonist. CRHR1 was found to be involved in trauma?induced anxiety. We then applied bioinformatic analysis to screen microRNAs (miRNAs or miRs) that target CRHR1, and miR?34b was determined to negatively regulate CRHR1 mRNA in primary hypothalamic neurons. The overexpression of miR?34b in the paraventricular nucleus (PVN) by a miRNA agomir using a drug delivery system decreased the hyperactivity of the HPA axis and anxiety?like behavior. Overall, the involvement of the HPA axis in trauma?induced anxiety was demonstrated, and trauma-induced anxiety was attenuated by decreasing the hyperactivity of the HPA axis via miR?34b by targeting CRHR1.

SUBMITTER: Zhu J 

PROVIDER: S-EPMC5466391 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature