Ontology highlight
ABSTRACT:
SUBMITTER: Igarashi Y
PROVIDER: S-EPMC5466583 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Igarashi Yuka Y Uchiyama Toru T Minegishi Tomoko T Takahashi Sirirat S Watanabe Nobuyuki N Kawai Toshinao T Yamada Masafumi M Ariga Tadashi T Onodera Masafumi M
Molecular therapy. Methods & clinical development 20170525
Clinical improvement in stem cell gene therapy (SCGT) for primary immunodeficiencies depends on the engraftment levels of genetically corrected cells, and tracing the transgene in each hematopoietic lineage is therefore extremely important in evaluating the efficacy of SCGT. We established a single cell-based droplet digital PCR (sc-ddPCR) method consisting of the encapsulation of a single cell into each droplet, followed by emulsion PCR with primers and probes specific for the transgene. A fluo ...[more]