MinION™ nanopore sequencing of environmental metagenomes: a synthetic approach.
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ABSTRACT:
SUBMITTER: Brown BL
PROVIDER: S-EPMC5467020 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
MinION™ nanopore sequencing of environmental metagenomes: a synthetic approach.
Brown Bonnie L BL Watson Mick M Minot Samuel S SS Rivera Maria C MC Franklin Rima B RB
GigaScience 20170301 3
Environmental metagenomic analysis is typically accomplished by assigning taxonomy and/or function from whole genome sequencing or 16S amplicon sequences. Both of these approaches are limited, however, by read length, among other technical and biological factors. A nanopore-based sequencing platform, MinION™, produces reads that are ≥1 × 104 bp in length, potentially providing for more precise assignment, thereby alleviating some of the limitations inherent in determining metagenome composition ...[more]
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