Dataset Information

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

ABSTRACT: The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.

SUBMITTER: Xie W

PROVIDER: S-EPMC5467035 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

Xie Wen W Chen Chunhai C Yang Zezhong Z Guo Litao L Yang Xin X Wang Dan D Chen Ming M Huang Jinqun J Wen Yanan Y Zeng Yang Y Liu Yating Y Xia Jixing J Tian Lixia L Cui Hongying H Wu Qingjun Q Wang Shaoli S Xu Baoyun B Li Xianchun X Tan Xinqiu X Ghanim Murad M Qiu Baoli B Pan Huipeng H Chu Dong D Delatte Helene H Maruthi M N MN Ge Feng F Zhou Xueping X Wang Xiaowei X Wan Fanghao F Du Yuzhou Y Luo Chen C Yan Fengming F Preisser Evan L EL Jiao Xiaoguo X Coates Brad S BS Zhao Jinyang J Gao Qiang Q Xia Jinquan J Yin Ye Y Liu Yong Y Brown Judith K JK Zhou Xuguo Joe XJ Zhang Youjun Y

GigaScience 20170501 5

The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using diffe ...[more]

PMID: 28327996

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Project description:BackgroundThe sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is one of the most widely distributed agricultural pests. Although it has developed resistance to many registered insecticides including the neonicotinoid insecticide thiamethoxam, the mechanisms that regulate the resistance are poorly understood. To understand the molecular basis of thiamethoxam resistance, "omics" analyses were carried out to examine differences between resistant and susceptible B. tabaci at both transcriptional and translational levels.ResultsA total of 1,338 mRNAs and 52 proteins were differentially expressed between resistant and susceptible B. tabaci. Among them, 11 transcripts had concurrent transcription and translation profiles. KEGG analysis mapped 318 and 35 differentially expressed genes and proteins, respectively, to 160 and 59 pathways (p<0.05). Thiamethoxam treatment activated metabolic pathways (e.g., drug metabolism), in which 118 transcripts were putatively linked to insecticide resistance, including up-regulated glutathione-S-transferase, UDP glucuronosyltransferase, glucosyl/glucuronosyl transferase, and cytochrome P450. Gene Ontology analysis placed these genes and proteins into protein complex, metabolic process, cellular process, signaling, and response to stimulus categories. Quantitative real-time PCR analysis validated "omics" response, and suggested a highly overexpressed P450, CYP6CX1, as a candidate molecular basis for the mechanistic study of thiamethoxam resistance in whiteflies. Finally, enzymatic activity assays showed elevated detoxification activities in the resistant B. tabaci.ConclusionsThis study demonstrates the applicability of high-throughput omics tools for identifying molecular candidates related to thiamethoxam resistance in an agricultural important insect pest. In addition, transcriptomic and proteomic analyses provide a solid foundation for future functional investigations into the complex molecular mechanisms governing the neonicotinoid resistance in whiteflies.

Dataset Information

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

Publications

Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

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