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CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion.

ABSTRACT: CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of ?-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of ?-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.

SUBMITTER: Mou H

PROVIDER: S-EPMC5470253 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion.

Mou Haiwei H Smith Jordan L JL Peng Lingtao L Yin Hao H Moore Jill J Zhang Xiao-Ou XO Song Chun-Qing CQ Sheel Ankur A Wu Qiongqiong Q Ozata Deniz M DM Li Yingxiang Y Anderson Daniel G DG Emerson Charles P CP Sontheimer Erik J EJ Moore Melissa J MJ Weng Zhiping Z Xue Wen W

Genome biology 20170614 1

CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larg ...[more]

PMID: 28615073

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