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Enrichment and fluorogenic labelling of 5-formyluracil in DNA.

ABSTRACT: Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to ?-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.

SUBMITTER: Liu C

PROVIDER: S-EPMC5472030 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Enrichment and fluorogenic labelling of 5-formyluracil in DNA.

Liu Chaoxing C Wang Yafen Y Zhang Xiong X Wu Fan F Yang Wei W Zou Guangrong G Yao Qian Q Wang Jiaqi J Chen Yuqi Y Wang Shaoru S Zhou Xiang X

Chemical science 20170405 6

Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated <i>o</i>-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property c ...[more]

PMID: 28660064

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