Ontology highlight
ABSTRACT:
SUBMITTER: Bacher R
PROVIDER: S-EPMC5473255 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Bacher Rhonda R Chu Li-Fang LF Leng Ning N Gasch Audrey P AP Thomson James A JA Stewart Ron M RM Newton Michael M Kendziorski Christina C
Nature methods 20170417 6
The normalization of RNA-seq data is essential for accurate downstream inference, but the assumptions upon which most normalization methods are based are not applicable in the single-cell setting. Consequently, applying existing normalization methods to single-cell RNA-seq data introduces artifacts that bias downstream analyses. To address this, we introduce SCnorm for accurate and efficient normalization of single-cell RNA-seq data. ...[more]