Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis.
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ABSTRACT:
SUBMITTER: Goswami PP
PROVIDER: S-EPMC5473781 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
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Recombinant 20.8-kDa protein of Mycobacterium avium subsp. paratuberculosis-based sero-diagnosis of paratuberculosis.
Goswami P P PP Chand Gokul G Prasad N S NS Deb R R Basagoudanavar S H SH
3 Biotech 20170616 3
Johne's disease or paratuberculosis is a chronic infectious enteric disease of ruminants caused by the intracellular pathogen. The control of the Johne's disease is hampered by lack of specific diagnostic tests. In this study, we have cloned and expressed the N-terminal region of the locus tag Map 1637c encoding 20.8-kDa (r20.8) protein of Mycobacterium avium subsp. paratuberculosis. The recombinant protein r20.8 was expressed in high levels in Escherichia coli. The protein r20.8 was purified by ...[more]
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