Dataset Information

Tunable Narrow Band Emissions from Dye-Sensitized Core/Shell/Shell Nanocrystals in the Second Near-Infrared Biological Window.

ABSTRACT: We introduce a hybrid organic-inorganic system consisting of epitaxial NaYF4:Yb3+/X3+@NaYbF4@NaYF4:Nd3+ (X = null, Er, Ho, Tm, or Pr) core/shell/shell (CSS) nanocrystal with organic dye, indocyanine green (ICG) on the nanocrystal surface. This system is able to produce a set of narrow band emissions with a large Stokes-shift (>200 nm) in the second biological window of optical transparency (NIR-II, 1000-1700 nm), by directional energy transfer from light-harvesting surface ICG, via lanthanide ions in the shells, to the emitter X3+ in the core. Surface ICG not only increases the NIR-II emission intensity of inorganic CSS nanocrystals by ?4-fold but also provides a broadly excitable spectral range (700-860 nm) that facilitates their use in bioapplications. We show that the NIR-II emission from ICG-sensitized Er3+-doped CSS nanocrystals allows clear observation of a sharp image through 9 mm thick chicken breast tissue, and emission signal detection through 22 mm thick tissue yielding a better imaging profile than from typically used Yb/Tm-codoped upconverting nanocrystals imaged in the NIR-I region (700-950 nm). Our result on in vivo imaging suggests that these ICG-sensitized CSS nanocrystals are suitable for deep optical imaging in the NIR-II region.

SUBMITTER: Shao W

PROVIDER: S-EPMC5474680 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Tunable Narrow Band Emissions from Dye-Sensitized Core/Shell/Shell Nanocrystals in the Second Near-Infrared Biological Window.

Shao Wei W Chen Guanying G Kuzmin Andrey A Kutscher Hilliard L HL Pliss Artem A Ohulchanskyy Tymish Y TY Prasad Paras N PN

Journal of the American Chemical Society 20161209 50

We introduce a hybrid organic-inorganic system consisting of epitaxial NaYF4:Yb3+/X3+@NaYbF4@NaYF4:Nd3+ (X = null, Er, Ho, Tm, or Pr) core/shell/shell (CSS) nanocrystal with organic dye, indocyanine green (ICG) on the nanocrystal surface. This system is able to produce a set of narrow band emissions with a large Stokes-shift (>200 nm) in the second biological window of optical transparency (NIR-II, 1000-1700 nm), by directional en ...[more]

PMID: 27935695

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Project description:Recently, the functionalization of nanoparticles, either within themselves or on the outer surface and its application in medicine, turned out to be the ultimate goal of nanotechnology. By providing these nanoparticles with chemical functional groups, one can force the nanoparticles to target the markers of the particular diseases or to measure the quantity and distribution of various intracellular species. In this paper, we report our development of a pH-responsive nanocomposite based on lanthanide-doped upconverting nanoparticles (UCNPs). Through multiphoton absorption and energy migration between spatially separated Nd3+, Yb3+, and Tm3+ in a three-layered NaYF4 host coated with FITC (fluorescein-5-isothiocyanate), this nanocomposite can measure the pH with high sensitivity. The fundamental acidity measurement is based on the pH-dependent equilibrium of the bright and dark states of FITC. The tremendous advantages of this system, regarding the pH measurement, come from the fact that the versatility of UCNP-imaging can fully be exploited. This includes the fact that (a) the optical wavelengths for the sensitization (980 nm and/or 808 nm) and the emission bands (UV, visible) are well separated, (b) the spectral overlap between FITC (absorption) and Tm3+ (emission) is substantially high, (c) there is no background signal due to the near-infrared laser, and (d) the signals are consistent regardless of the fluctuations by monitoring the ratio of blue band with respect to the unaffected self-reference (red and near-infrared bands). Moreover, the double shell structure is obviously superior to the core-shell structure in that it enhances the spectral separation between the sensitizer and the emitter in the upconversion process, inhibiting any unnecessary contamination in the spectra. Finally, it is noteworthy that Yb3+ plays crucial roles as a sensitizer at 980 nm excitation and a bridge above which 808 nm excitation migrates from Nd3+ to Tm3+ via the Yb3+ excited state.

Dataset Information

Tunable Narrow Band Emissions from Dye-Sensitized Core/Shell/Shell Nanocrystals in the Second Near-Infrared Biological Window.

Publications

Tunable Narrow Band Emissions from Dye-Sensitized Core/Shell/Shell Nanocrystals in the Second Near-Infrared Biological Window.

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