Variables influencing fluorimetric N-sulfoglucosamine sulfohydrolase (SGSH) activity measurement in brain homogenates.
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ABSTRACT:
SUBMITTER: Whyte LS
PROVIDER: S-EPMC5475436 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
Variables influencing fluorimetric <i>N</i>-sulfoglucosamine sulfohydrolase (SGSH) activity measurement in brain homogenates.
Whyte Lauren S LS Hopwood John J JJ Hemsley Kim M KM Lau Adeline A AA
Molecular genetics and metabolism reports 20151022
Deficient <i>N</i>-sulfoglucosamine sulfohydrolase (SGSH) enzyme activity causes mucopolysaccharidosis (MPS) type IIIA. A fluorimetric SGSH activity assay is commonly used to examine patient cells. Here, we modified this method for brain homogenates and define the parameters for assay linearity. SGSH activity was suppressed outside of these parameters. This method will enable the accurate measurement of SGSH activity in MPS IIIA tissues to examine disease pathogenesis and evaluate therapies. ...[more]
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