Replacing a Century Old Technique - Modern Spectroscopy Can Supplant Gram Staining.
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ABSTRACT:
SUBMITTER: Berezin S
PROVIDER: S-EPMC5476599 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Publications
Replacing a Century Old Technique - Modern Spectroscopy Can Supplant Gram Staining.
Berezin Shirly S Aviv Yaron Y Aviv Hagit H Goldberg Elad E Tischler Yaakov R YR
Scientific reports 20170619 1
Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enha ...[more]
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