Ontology highlight
ABSTRACT:
SUBMITTER: Xie C
PROVIDER: S-EPMC5478605 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Xie Cong C Yan Tong-Meng TM Chen Jia-Mei JM Li Xiao-Yan XY Zou Juan J Zhu Li-Jun LJ Lu Lin-Lin LL Wang Ying Y Zhou Fu-Yuan FY Liu Zhong-Qiu ZQ Hu Ming M
Scientific reports 20170620 1
This study aims to determine whether enzyme activities are correlated with protein amounts and mRNA expression levels of five major human sulfotransferase (SULT) enzymes in 10 matched pericarcinomatous and hepatocellular carcinoma liver samples. The MRM UHPLC-MS/MS method, Western blot and RT-PCR were used along with SULT activity measurement using probe substrates. The LC-MS/MS method was specific for all five tested SULTs, whereas Western blot was specific for only two isoforms. The activities ...[more]