Novel siRNA formulation to effectively knockdown mutant p53 in osteosarcoma.

ABSTRACT:

Objectives

The tumor suppressor p53 plays a crucial role in the development of osteosarcoma. The primary objective of this study is to develop and optimize lipid based nanoparticle formulations that can carry siRNA and effectively silence mutant p53 in 318-1, a murine osteosarcoma cell line.

Methods

The nanoparticles were composed of a mixture of two lipids (cholesterol and DOTAP) and either PLGA or PLGA-PEG and prepared by using an EmulsiFlex-B3 high pressure homogenizer. A series of studies that include using different nanoparticles, different amount of siRNAs, cell numbers, incubation time, transfection media volume, and storage temperature was performed to optimize the gene silencing efficiency.

Key findings

Replacement of lipids by PLGA or PLGA-PEG decreased the

SUBMITTER: Kundu AK 

PROVIDER: S-EPMC5479560 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature