Stable centromere positioning in diverse sequence contexts of complex and satellite centromeres of maize and wild relatives.

ABSTRACT:

Background

Paradoxically, centromeres are known both for their characteristic repeat sequences (satellite DNA) and for being epigenetically defined. Maize (Zea mays mays) is an attractive model for studying centromere positioning because many of its large (~2 Mb) centromeres are not dominated by satellite DNA. These centromeres, which we call complex centromeres, allow for both assembly into reference genomes and for mapping short reads from ChIP-seq with antibodies to centromeric histone H3 (cenH3).

Results

We found frequent complex centromeres in maize and its wild relatives Z. mays parviglumis, Z. mays mexicana, and particularly Z. mays huehuetenangensis. Analysis of individual plants reveals minor variation in the positions of complex centromeres among siblings. However,

SUBMITTER: Gent JI 

PROVIDER: S-EPMC5480163 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature