Ontology highlight
ABSTRACT:
SUBMITTER: Dedigama-Arachchige PM
PROVIDER: S-EPMC5481203 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Dedigama-Arachchige Pavithra M PM Pflum Mary Kay H MK
ACS chemical biology 20161017 12
Few methods are available to discover the cellular kinase that phosphorylates a specific amino acid, or phosphosite, on a protein. In addition, identifying the associated proteins bound near a phosphosite during phosphorylation would provide insights into cell biology and signaling. Here, we report K-CLASP (Kinase Catalyzed CrossLinking And Streptavidin Purification) as a method for both phosphosite-specific kinase identification and the discovery of kinase interacting proteins. K-CLASP offers a ...[more]