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Predicting conformational ensembles and genome-wide transcription factor binding sites from DNA sequences.

ABSTRACT: DNA shape is emerging as an important determinant of transcription factor binding beyond just the DNA sequence. The only tool for large scale DNA shape estimates, DNAshape was derived from Monte-Carlo simulations and predicts four broad and static DNA shape features, Propeller twist, Helical twist, Minor groove width and Roll. The contributions of other shape features e.g. Shift, Slide and Opening cannot be evaluated using DNAshape. Here, we report a novel method DynaSeq, which predicts molecular dynamics-derived ensembles of a more exhaustive set of DNA shape features. We compared the DNAshape and DynaSeq predictions for the common features and applied both to predict the genome-wide binding sites of 1312 TFs available from protein interaction quantification (PIQ) data. The results indicate a good agreement between the two methods for the common shape features and point to advantages in using DynaSeq. Predictive models employing ensembles from individual conformational parameters revealed that base-pair opening - known to be important in strand separation - was the best predictor of transcription factor-binding sites (TFBS) followed by features employed by DNAshape. Of note, TFBS could be predicted not only from the features at the target motif sites, but also from those as far as 200 nucleotides away from the motif.

SUBMITTER: Andrabi M

PROVIDER: S-EPMC5481346 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Predicting conformational ensembles and genome-wide transcription factor binding sites from DNA sequences.

Andrabi Munazah M Hutchins Andrew Paul AP Miranda-Saavedra Diego D Kono Hidetoshi H Nussinov Ruth R Mizuguchi Kenji K Ahmad Shandar S

Scientific reports 20170622 1

DNA shape is emerging as an important determinant of transcription factor binding beyond just the DNA sequence. The only tool for large scale DNA shape estimates, DNAshape was derived from Monte-Carlo simulations and predicts four broad and static DNA shape features, Propeller twist, Helical twist, Minor groove width and Roll. The contributions of other shape features e.g. Shift, Slide and Opening cannot be evaluated using DNAshape. Here, we report a novel method DynaSeq, which predicts molecula ...[more]

PMID: 28642456

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Dataset Information

Predicting conformational ensembles and genome-wide transcription factor binding sites from DNA sequences.

Publications

Predicting conformational ensembles and genome-wide transcription factor binding sites from DNA sequences.

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