Dataset Information

PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/?-catenin signaling pathway.

ABSTRACT:

Background

Protein regulator of cytokinesis-1 (PRC1) belongs to the microtubule-associated proteins (MAPs) family, and is involved in cytokinesis. Recent investigations suggest PRC1 involvement in human carcinogenesis, including breast carcinoma, hepatocellular carcinoma and etc. However, whether PRC1 contributes to lung adenocarcinoma tumorigenesis remains unknown.

Methods

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blotting and Immunohistochemical staining (IHC) were used to evaluate and contrast the PRC1 expression profile in lung adenocarcinoma and adjacent normal lung tissues. We examined the clinical use of PRC1 in lung adenocarcinoma prognosis. Additionally, the tumorigenesis impact of PRC1 in lung adenocarcinoma cells was verified via in vitro and in vivo metastasis and tumorigenesis assays. Notably, Next Generation Sequencing (NGS) was performed to investigate the molecular mechanism underlying the oncogenic role of PRC1 in lung adenocarcinoma.

Results

PRC1 mRNA and protein expressions were upregulated in lung adenocarcinoma tissues compared to adjacent normal lung tissues. PRC1 protein overexpression correlated with lymph node metastasis and was an independent poor prognostic factor for lung adenocarcinoma patients. Our data implied that PRC1 depletion limited the proliferation and invasion of lung adenocarcinoma cells in vitro and lowered tumor development and lung metastasis in vivo. Remarkably, limiting PRC1 substantially prompted G2/M phase cell cycle arrest and apoptosis. Mechanistically, by conducting NGS on PRC1-depleted A549 cells and control cells, we discovered that PRC1 expression was significantly correlated with the Wnt signaling pathway.

Conclusions

This investigation offers confirmation that PRC1 is a prognostic and promising therapeutic biomarker for people with lung adenocarcinoma and takes on a key part in the activation of the Wnt/?-catenin pathway in lung adenocarcinoma development.

SUBMITTER: Zhan P

PROVIDER: S-EPMC5483280 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Publications

PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway.

Zhan Ping P Zhang Bin B Xi Guang-Min GM Wu Ying Y Liu Hong-Bing HB Liu Ya-Fang YF Xu Wu-Jian WJ Zhu Qing-Qing QQ Cai Feng F Zhou Ze-Jun ZJ Miu Ying-Ying YY Wang Xiao-Xia XX Jin Jia-Jia JJ Li Qian Q Qian Li-Ping LP Lv Tang-Feng TF Song Yong Y

Molecular cancer 20170624 1

<h4>Background</h4>Protein regulator of cytokinesis-1 (PRC1) belongs to the microtubule-associated proteins (MAPs) family, and is involved in cytokinesis. Recent investigations suggest PRC1 involvement in human carcinogenesis, including breast carcinoma, hepatocellular carcinoma and etc. However, whether PRC1 contributes to lung adenocarcinoma tumorigenesis remains unknown.<h4>Methods</h4>Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blotting and Immunohistochem ...[more]

PMID: 28646916

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