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Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII.

ABSTRACT: During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.

SUBMITTER: Musiol-Kroll EM

PROVIDER: S-EPMC5483334 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII.

Musiol-Kroll Ewa M EM Zubeil Florian F Schafhauser Thomas T Härtner Thomas T Kulik Andreas A McArthur John J Koryakina Irina I Wohlleben Wolfgang W Grond Stephanie S Williams Gavin J GJ Lee Sang Yup SY Weber Tilmann T

ACS synthetic biology 20170222 3

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by ...[more]

PMID: 28206741

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