Unknown

Dataset Information

0

The Drosophila mitochondrial ribosomal protein mRpL12 is required for Cyclin D/Cdk4-driven growth.


ABSTRACT: The Drosophila melanogaster cyclin-dependent protein kinase complex CycD/Cdk4 stimulates both cell cycle progression and cell growth (accumulation of mass). CycD/Cdk4 promotes cell cycle progression via the well-characterized RBF/E2F pathway, but our understanding of how growth is stimulated is still limited. To identify growth regulatory targets of CycD/Cdk4, we performed a loss-of-function screen for modifiers of CycD/Cdk4-induced overgrowth of the Drosophila eye. One mutation that suppressed CycD/Cdk4 was in a gene encoding the mitochondrial ribosomal protein, mRpL12. We show here that mRpL12 is required for CycD/Cdk4-induced cell growth. Cells homozygous mutant for mRpL12 have reduced mitochondrial activity, and exhibit growth defects that are very similar to those of cdk4 null cells. CycD/Cdk4 stimulates mitochondrial activity, and this is mRpL12 dependent. Hif-1 prolyl hydroxylase (Hph), another effector of CycD/Cdk4, regulates growth and is required for inhibition of the hypoxia-inducible transcription factor 1 (Hif-1). Both functions depend on mRpL12 dosage, suggesting that CycD/Cdk4, mRpL12 and Hph function together in a common pathway that controls cell growth via affecting mitochondrial activity.

SUBMITTER: Frei C 

PROVIDER: S-EPMC548645 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Drosophila mitochondrial ribosomal protein mRpL12 is required for Cyclin D/Cdk4-driven growth.

Frei Christian C   Galloni Mireille M   Hafen Ernst E   Edgar Bruce A BA  

The EMBO journal 20050203 3


The Drosophila melanogaster cyclin-dependent protein kinase complex CycD/Cdk4 stimulates both cell cycle progression and cell growth (accumulation of mass). CycD/Cdk4 promotes cell cycle progression via the well-characterized RBF/E2F pathway, but our understanding of how growth is stimulated is still limited. To identify growth regulatory targets of CycD/Cdk4, we performed a loss-of-function screen for modifiers of CycD/Cdk4-induced overgrowth of the Drosophila eye. One mutation that suppressed  ...[more]

Similar Datasets

| S-EPMC302080 | biostudies-literature
| S-EPMC2735006 | biostudies-literature
| S-EPMC3315095 | biostudies-literature
| S-EPMC5105351 | biostudies-literature
| S-EPMC1890844 | biostudies-other
| S-EPMC302073 | biostudies-literature
| S-EPMC6464582 | biostudies-literature
| S-EPMC2864756 | biostudies-literature
2018-10-20 | GSE121533 | GEO
| S-EPMC3787750 | biostudies-literature