Relevant principal factors affecting the reproducibility of insect primary culture.
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ABSTRACT:
SUBMITTER: Ogata N
PROVIDER: S-EPMC5486689 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Publications
Relevant principal factors affecting the reproducibility of insect primary culture.
Ogata Norichika N Iwabuchi Kikuo K
In vitro cellular & developmental biology. Animal 20170222 6
The primary culture of insect cells often suffers from problems with poor reproducibility in the quality of the final cell preparations. The cellular composition of the explants (cell number and cell types), surgical methods (surgical duration and surgical isolation), and physiological and genetic differences between donors may be critical factors affecting the reproducibility of culture. However, little is known about where biological variation (interindividual differences between donors) ends ...[more]
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