Ontology highlight
ABSTRACT:
SUBMITTER: Peng S
PROVIDER: S-EPMC5488182 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20170511 24
Single-molecule fluorescence resonance energy transfer (sm-FRET) has become a widely used tool to reveal dynamic processes and molecule mechanisms hidden under ensemble measurements. However, the upper limit of fluorescent species used in sm-FRET is still orders of magnitude lower than the association affinity of many biological processes under physiological conditions. Herein, we introduce single-molecule photoactivation FRET (sm-PAFRET), a general approach to break the concentration barrier by ...[more]