Ontology highlight
ABSTRACT:
SUBMITTER: Westley C
PROVIDER: S-EPMC5488198 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Westley Chloe C Fisk Heidi H Xu Yun Y Hollywood Katherine A KA Carnell Andrew J AJ Micklefield Jason J Turner Nicholas J NJ Goodacre Royston R
Chemistry (Weinheim an der Bergstrasse, Germany) 20170502 29
For enzyme-catalysed biotransformations, continuous in situ detection methods minimise the need for sample manipulation, ultimately leading to more accurate real-time kinetic determinations of substrate(s) and product(s). We have established for the first time an on-line, real-time quantitative approach to monitor simultaneously multiple biotransformations based on UV resonance Raman (UVRR) spectroscopy. To exemplify the generality and versatility of this approach, multiple substrates and enzyme ...[more]