Dataset Information

Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens.

ABSTRACT: Sandwich ELISA methods have been widely used for biomarker and pathogen detection because of their high specificity and sensitivity. However, the main drawbacks of this assay are the cost, the time-consuming procedure for the isolation of antibodies and their poor stability. To overcome these restrictions, we herein fabricated artificial antibodies based on imprinting technology and developed a sandwich ELISA for pathogen detection. Both the capture and detection antibodies were obtained via an in situ method, with simplicity, rapidity and low cost. The peroxidase mimics, the CeO₂ nanoparticles, as signal generators were integrated with the detection antibody. The fabricated artificial antibodies exhibited not only natural antibody-like binding affinities and selectivities, but also superior stability and reusability. The detection limit was about 500 CFU mL^-1, which is much lower than that of traditional ELISA methods (10⁴ to 10⁵ CFU mL^-1). Furthermore, the capture antibody can disinfect pathogens in situ.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC5489049 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Highly stable and reusable imprinted artificial antibody used for <i>in situ</i> detection and disinfection of pathogens.

Zhang Zhijun Z Guan Yijia Y Li Meng M Zhao Andong A Ren Jinsong J Qu Xiaogang X

Chemical science 20150223 5

Sandwich ELISA methods have been widely used for biomarker and pathogen detection because of their high specificity and sensitivity. However, the main drawbacks of this assay are the cost, the time-consuming procedure for the isolation of antibodies and their poor stability. To overcome these restrictions, we herein fabricated artificial antibodies based on imprinting technology and developed a sandwich ELISA for pathogen detection. Both the capture and detection antibodies were obtained <i>via< ...[more]

PMID: 28706671

Similar Datasets

Project description:BACKGROUND:This study is to elucidate the disinfection effect of ozone producing low-pressure Hg vapor lamps against human pathogens. Ozone producing low-pressure Hg vapor lamps emit mainly 254?nm ultraviolet light C (UVC) with about 10% power of Vacuum-ultraviolet (VUV) light at 185?nm. The combination of UVC and VUV can inactivate airborne pathogens by disrupting the genetic materials or generation of reactive oxygen species, respectively. In this study, inactivation of common bacteria including Escherichia coli ATCC25922 (E. coli), Extended Spectrum Beta-Lactamase-producing E. coli (ESBL), Methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis (MTB), and that of influenza A viruses H1N1 and H3N2 under the radiation from ozone producing low-pressure Hg vapor lamps was examined. Log reduction values at different treatment durations were determined. METHODS:In vitro tests were carried out. Various bacterium and virus suspensions were added onto nitrocellulose filter papers and subjected to the illumination from ozone producing low-pressure Hg vapor lamps. The extents of pathogen inactivation at different illumination times were investigated by conducting a series of experiments with increasing duration of illumination. log10 reduction in CFU/ml and reduction at log10(TCID50) were respectively measured for bacteria and viruses. The disinfection effectiveness of this type of lamps against the pathogens under the environment with a moderate barrier to light was therefore evaluated. RESULTS:Ozone producing low-pressure Hg vapor lamp successfully inactivated these human pathogens. Nevertheless, among these pathogens, disinfection of MTB required more intense treatment. In the best tested situation, 3-log10 inactivation of pathogens can be achieved with ?10?min of VUV treatment except MTB which needed about 20?min. This demonstrated the high resistance against UV disinfection of MTB. CONCLUSIONS:Following the criteria that valid germicidal results can be reflected with 3-log10 inactivation for bacteria, 4-log10 inactivation for viruses and 5-log10 inactivation for MTB, most of the bacteria required ?10?min of VUV treatment, 20?min for the influenza viruses while MTB needed about 30?min VUV treatment. This indicated that VUV light is an effective approach against different environmental microorganisms.

Dataset Information

Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens.

Publications

Highly stable and reusable imprinted artificial antibody used for <i>in situ</i> detection and disinfection of pathogens.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets