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Immunization with Toxoplasma gondii GRA17 Deletion Mutant Induces Partial Protection and Survival in Challenged Mice.

ABSTRACT: Toxoplasmosis remains a world-threatening disease largely because of the lack of a fully effective vaccine. Here, we created a ?GRA17 mutant by disrupting the virulence factor GRA17 using CRISPR-Cas9 method. Then, we tested whether ?GRA17 tachyzoites can be used as a live-attenuated vaccine against acute, chronic, and congenital Toxoplasma gondii infection in mice. Immune response evoked by ?GRA17 immunization suggested a sequential Th1 and Th2 T cell response, indicated by high levels of Th1 and a mixed Th1/Th2 cytokines at 28 and 70?days after immunization, respectively. ?GRA17-mediated immunity fully protected mice against lethal infection with wild-type (wt) RH strain, heterologous challenge with PYS, and TgC7 strains of the Chinese ToxoDB#9 genotype, and T. gondii Pru strain. Although parasite cysts were detected in 8 out of 10 immunized mice, cyst burden in the brain was significantly reduced (P?

SUBMITTER: Wang JL 

PROVIDER: S-EPMC5489627 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Immunization with <i>Toxoplasma gondii GRA17</i> Deletion Mutant Induces Partial Protection and Survival in Challenged Mice.

Wang Jin-Lei JL   Elsheikha Hany M HM   Zhu Wei-Ning WN   Chen Kai K   Li Ting-Ting TT   Yue Dong-Mei DM   Zhang Xiao-Xuan XX   Huang Si-Yang SY   Zhu Xing-Quan XQ  

Frontiers in immunology 20170629

Toxoplasmosis remains a world-threatening disease largely because of the lack of a fully effective vaccine. Here, we created a Δ<i>GRA17</i> mutant by disrupting the virulence factor <i>GRA17</i> using CRISPR-Cas9 method. Then, we tested whether Δ<i>GRA17</i> tachyzoites can be used as a live-attenuated vaccine against acute, chronic, and congenital <i>Toxoplasma gondii</i> infection in mice. Immune response evoked by Δ<i>GRA17</i> immunization suggested a sequential Th1 and Th2 T cell response,  ...[more]

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