Unknown

Dataset Information

0

Hypoxia-inducible factor-1? promotes endometrial stromal cells migration and invasion by upregulating autophagy in endometriosis.


ABSTRACT: Endometriosis is a benign gynecological disease that shares some characteristics with malignancy like migration and invasion. It has been reported that both hypoxia-inducible factor-1? (HIF-1?) and autophagy were upregulated in ectopic endometrium of patients with ovarian endometriosis. However, the crosstalk between HIF-1? and autophagy in the pathogenesis of endometriosis remains to be clarified. Accordingly, we investigated whether autophagy was regulated by HIF-1?, as well as whether the effect of HIF-1? on cell migration and invasion is mediated through autophagy upregulation. Here, we found that ectopic endometrium from patients with endometriosis highly expressed HIF-1? and autophagy-related protein LC3. In cultured human endometrial stromal cells (HESCs), autophagy was induced by hypoxia in a time-dependent manner and autophagy activation was dependent on HIF-1?. In addition, migration and invasion ability of HESCs were enhanced by hypoxia treatment, whereas knockdown of HIF-1? attenuated this effect. Furthermore, inhibiting autophagy with specific inhibitors and Beclin1 siRNA attenuated hypoxia triggered migration and invasion of HESCs. Taken together, these results suggest that HIF-1? promotes HESCs invasion and metastasis by upregulating autophagy. Thus, autophagy may be involved in the pathogenesis of endometriosis and inhibition of autophagy might be a novel therapeutic approach to the treatment of endometriosis.

SUBMITTER: Liu H 

PROVIDER: S-EPMC5489654 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Hypoxia-inducible factor-1α promotes endometrial stromal cells migration and invasion by upregulating autophagy in endometriosis.

Liu Hengwei H   Zhang Zhibing Z   Xiong Wenqian W   Zhang Ling L   Xiong Yao Y   Li Na N   He Haitang H   Du Yu Y   Liu Yi Y  

Reproduction (Cambridge, England) 20170327 6


Endometriosis is a benign gynecological disease that shares some characteristics with malignancy like migration and invasion. It has been reported that both hypoxia-inducible factor-1α (HIF-1α) and autophagy were upregulated in ectopic endometrium of patients with ovarian endometriosis. However, the crosstalk between HIF-1α and autophagy in the pathogenesis of endometriosis remains to be clarified. Accordingly, we investigated whether autophagy was regulated by HIF-1α, as well as whether the eff  ...[more]

Similar Datasets

| S-EPMC9588377 | biostudies-literature
| S-EPMC4748282 | biostudies-literature
| S-EPMC10308507 | biostudies-literature
| S-EPMC6307811 | biostudies-literature
| S-EPMC9661757 | biostudies-literature
| S-EPMC3094047 | biostudies-literature
| S-EPMC5780386 | biostudies-literature
| S-EPMC9761153 | biostudies-literature
| S-EPMC8806971 | biostudies-literature
| S-EPMC6592818 | biostudies-literature