Unknown

Dataset Information

0

Mammalian Innate Immune Response to a Leishmania-Resident RNA Virus Increases Macrophage Survival to Promote Parasite Persistence.


ABSTRACT: Some strains of the protozoan parasite Leishmania guyanensis (L.g) harbor a viral endosymbiont called Leishmania RNA virus 1 (LRV1). LRV1 recognition by TLR-3 increases parasite burden and lesion swelling in vivo. However, the mechanisms by which anti-viral innate immune responses affect parasitic infection are largely unknown. Upon investigating the mammalian host's response to LRV1, we found that miR-155 was singularly and strongly upregulated in macrophages infected with LRV1+ L.g when compared to LRV1- L.g. LRV1-driven miR-155 expression was dependent on TLR-3/TRIF signaling. Furthermore, LRV1-induced TLR-3 activation promoted parasite persistence by enhancing macrophage survival through Akt activation in a manner partially dependent on miR-155. Pharmacological inhibition of Akt resulted in a decrease in LRV1-mediated macrophage survival and consequently decreased parasite persistence. Consistent with these data, miR-155-deficient mice showed a drastic decrease in LRV1-induced disease severity, and lesional macrophages from these mice displayed reduced levels of Akt phosphorylation.

SUBMITTER: Eren RO 

PROVIDER: S-EPMC5493041 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mammalian Innate Immune Response to a Leishmania-Resident RNA Virus Increases Macrophage Survival to Promote Parasite Persistence.

Eren Remzi Onur RO   Reverte Marta M   Rossi Matteo M   Hartley Mary-Anne MA   Castiglioni Patrik P   Prevel Florence F   Martin Ricardo R   Desponds Chantal C   Lye Lon-Fye LF   Drexler Stefan K SK   Reith Walter W   Beverley Stephen M SM   Ronet Catherine C   Fasel Nicolas N  

Cell host & microbe 20160901 3


Some strains of the protozoan parasite Leishmania guyanensis (L.g) harbor a viral endosymbiont called Leishmania RNA virus 1 (LRV1). LRV1 recognition by TLR-3 increases parasite burden and lesion swelling in vivo. However, the mechanisms by which anti-viral innate immune responses affect parasitic infection are largely unknown. Upon investigating the mammalian host's response to LRV1, we found that miR-155 was singularly and strongly upregulated in macrophages infected with LRV1+ L.g when compar  ...[more]

Similar Datasets

| S-EPMC5078497 | biostudies-literature
| S-EPMC4102420 | biostudies-literature
| S-EPMC10577668 | biostudies-literature
| S-EPMC3186911 | biostudies-literature
| S-EPMC2722086 | biostudies-other
| S-EPMC3861331 | biostudies-literature
| S-EPMC3916414 | biostudies-literature
| S-EPMC3659421 | biostudies-literature
| S-EPMC5996280 | biostudies-literature
| S-EPMC5004886 | biostudies-literature