Mapping of B-cell epitopes in a Trypanosoma cruzi immunodominant antigen expressed in natural infections.
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ABSTRACT:
SUBMITTER: Lesenechal M
PROVIDER: S-EPMC549311 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
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Mapping of B-cell epitopes in a Trypanosoma cruzi immunodominant antigen expressed in natural infections.
Lesénéchal Mylène M Becquart Laurence L Lacoux Xavier X Ladavière Laurent L Baida Renata C P RC Paranhos-Baccalà Glaucia G da Silveira José Franco JF
Clinical and diagnostic laboratory immunology 20050201 2
Tc40 is an immunodominant antigen present in natural Trypanosoma cruzi infections. This immunogen was thoroughly mapped by using overlapping amino acid sequences identified by gene cloning and chemical peptide synthesis. To map continuous epitopes of the Tc40 antigen, an epitope expression library was constructed and screened with sera from human chagasic patients. A major, linear B-cell epitope spanning residues 403 to 426 (PAKAAAPPAA) was identified in the central domain of Tc40. A synthetic p ...[more]
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