Ontology highlight
ABSTRACT:
SUBMITTER: Yang YN
PROVIDER: S-EPMC5493576 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Yang Yi-Nan YN Shan Wen-Xin WX Wang Pi-Wu PW
3 Biotech 20170630 3
Cyclodextrin glucanotransferase (CGTase) is an important enzyme with multiple functions in starch utilization. In the present study, a fermentation process for the production of CGTase from Escherichia coli harboring the recombinant plasmid pET28b(+)-CGTase was investigated and optimized. The optimal fermentation and expression conditions were 10.0 g/L glycerol, 20.0 g/L tryptone, and 10.0 g/L yeast extract with an initial pH of 7.0, an IPTG concentration of 0.1 mM and an induction temperature o ...[more]