The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding.
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ABSTRACT:
SUBMITTER: Leeder WM
PROVIDER: S-EPMC5494072 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
The 2D Structure of the <i>T. brucei</i> Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding.
Leeder W-Matthias WM Voskuhl Stephan S Göringer H Ulrich HU
Journal of nucleic acids 20170618
Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether these structures resemble the in vivo folds given the extreme "crowding" conditions within the mitochondrion. Here, we analyze the effects of macromolecular crowding on the structure of the mitochondrial RPS12 pre-mRNA. We use high molecular mass p ...[more]
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