Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck.

ABSTRACT:

Objective

The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds.

Methods

Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established.

Results

The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high

SUBMITTER: Luo J 

PROVIDER: S-EPMC5495669 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature