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Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities.

ABSTRACT: Purple coloured tea shoot clones have gained interest due to high content of anthocyanins in addition to catechins. Transcript expression of genes encoding anthocyanidin reductase (ANR), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), flavonol synthase (FLS) and leucoantho cyanidin reductase (LAR) enzymes in three new purple shoot tea clones compared with normal tea clone showed higher expression of CsDFR, CsANR, CsANS and lower expression of CsFLS and CsLAR in purple shoot clones compared to normal clone. Expression pattern supported high content of anthocyanins in purple tea. Four anthocyanins (AN_1-4) were isolated and characterized by UPLC-ESI-QToF-MS/MS from IHBT 269 clone which recorded highest total anthocyanins content. Cyanidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN₂) showed highest in vitro antioxidant activity (IC_50 DPPH = 25.27 ± 0.02 ?g/mL and IC_50 ABTS = 10.71 ± 0.01 ?g/mL). Anticancer and immunostimulatory activities of cyanidin-3-glucoside (AN₁), cyanidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN₂), delphinidin-3-O-?-d-(6-(E)-coumaroyl) glucopyranoside (AN₃), cyanidin-3-O-(2-O-?-xylopyranosyl-6-O-acetyl)-?-glucopyranoside (AN₄) and crude anthocyanin extract (AN₅) showed high therapeutic perspective. Anthocyanins AN_1-4 and crude extract AN₅ showed cytotoxicity on C-6 cancer cells and high relative fluorescence units (RFU) at 200 ?g/mL suggesting promising apoptosis induction activity as well as influential immunostimulatory potential. Observations demonstrate potential of purple anthocyanins enriched tea clone for exploitation as a nutraceutical product.

SUBMITTER: Joshi R

PROVIDER: S-EPMC5495722 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities.

Joshi Robin R Rana Ajay A Kumar Vinay V Kumar Dharmesh D Padwad Yogendra S YS Yadav Sudesh Kumar SK Gulati Ashu A

Journal of food science and technology 20170418 7

Purple coloured tea shoot clones have gained interest due to high content of anthocyanins in addition to catechins. Transcript expression of genes encoding anthocyanidin reductase (ANR), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), flavonol synthase (FLS) and leucoantho cyanidin reductase (LAR) enzymes in three new purple shoot tea clones compared with normal tea clone showed higher expression of CsDFR, CsANR, CsANS and ...[more]

PMID: 28720952

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Project description:Previous studies demonstrated that enzymatic hydrolysis enhances wheat bran (WB) biological properties. This study evaluated the immunostimulatory effect of a WB hydrolysate (HYD) and a mousse enriched with HYD (MH) before and after in vitro digestion on murine and human macrophages. The antiproliferative activity of the harvested macrophage supernatant on colorectal cancer (CRC) cells was also analyzed. MH showed significantly higher content than control mousse (M) in soluble poly- and oligosaccharides (OLSC), as well as total soluble phenolic compounds (TSPC). Although in vitro gastrointestinal digestion slightly reduced the TSPC bioaccessibility of MH, ferulic acid (FA) levels remained stable. HYD showed the highest antioxidant activity followed by MH, which demonstrated a greater antioxidant activity before and after digestion as compared with M. RAW264.7 and THP-1 cells released the highest amounts of pro-inflammatory cytokines after being treated with 0.5 mg/mL of digested WB samples. Treatment with digested HYD-stimulated RAW264.7 supernatant for 96 h showed the most anticancer effect, and spent medium reduced cancer cell colonies more than direct WB sample treatments. Although a lack of inner mitochondrial membrane potential alteration was found, increased Bax:Bcl-2 ratio and caspase-3 expression suggested activation of the mitochondrial apoptotic pathway when CRC cells were treated with macrophage supernatants. Intracellular reactive oxygen species (ROS) were positively correlated with the cell viability in CRC cells exposed to RAW264.7 supernatants (r = 0.78, p < 0.05) but was not correlated in CRC cells treated with THP-1 conditioned media. Supernatant from WB-stimulated THP-1 cells may be able to stimulate ROS production in HT-29 cells, leading to a decrease of viable cells in a time-dependent manner. Therefore, our present study revealed a novel anti-tumour mechanism of HYD through the stimulation of cytokine production in macrophages and the indirect inhibition of cell proliferation, colony formation, and activation of pro-apoptotic proteins expression in CRC cells.

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Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities.

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Anthocyanins enriched purple tea exhibits antioxidant, immunostimulatory and anticancer activities.

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